Title : Genetic diversity and molecular epidemiology of mycobacterium tuberculosis from pleural specimen in Morocco
Abstract:
Objectives:
Development of pleural form of tuberculosis is still poorly understood, particularly in quite high-endemic countries like Morocco. Genotypic studies may help understanding the transmission routes of Mycobacterium tuberculosis strains in pleural TB patients.
Material and Methods: Among 156 new pleural TB cases, 28 were culture positive (17.9%). 7 distinct spoligotypes belonged to four spoligotype families: Latin-American & Mediterranean (LAM) (13/28) of the isolates, Haarlem (H) (10/28), and T group (T) (2/28), and Beijing (3/28). MIRU-VNTR typing subdivided the largest spoligotype cluster, and defined a total of 3 clustered isolates.
Conclusions: Within the study limits, these proportions were similar to those found among pulmonary TB (PTB) cases in Morocco. Of note, the three (03) multi-drug (MDR) resistant isolate were Beijing type, exactly matching the 24-locus type of a major European-Russian Beijing MDR clone.
Key words: Mycobacterium Tuberculosis, Extrapulmonary-TB, resistance, spoligotyping, 24-locus based MIRU-VNTR typing.
