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4th Edition of World Congress on Infectious Diseases

June 21-22, 2023 | Rome, Italy

June 21 -22, 2023 | Rome, Italy
Infection 2023

Chin Kim Ling

Speaker at World Congress on Infectious Diseases 2023 - Chin Kim Ling
Institute for Advanced Studies, Universiti Malaya, Malaysia
Title : High mobility group box 1: A novel host restriction factor in zika virus replication

Abstract:

Neonatal microcephaly and adult Guillain-Barré syndrome are severe complications of Zika virus (ZIKV) infection. The robustly induced inflammatory cytokine expressions in ZIKV-infected patients may constitute a hallmark for severe disease. One of them is the high mobility group box 1 protein (HMGB1). This study investigated the involvement of HMGB1 and its potential role in ZIKV infection of Huh7 cells. HMGB1 protein expression was analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blot. Meanwhile, HMGB1’s role in ZIKV infection was explored using resveratrol, a natural polyphenol, and HMGB1-knockdown cells. Results of focus-forming assay showed that Huh7 cells were highly susceptible to ZIKV replication. ZIKV infection was found to induce HMGB1 nuclear-to-cytoplasmic translocation, resulting in a >100-fold increase in cytosol HMGB1 expression at 72h.p.i. Consistently, the extracellular HMGB1 production induced by ZIKV infection was elevated in a time- and multiplicity of infection (MOI)- dependent manner. Resveratrol treatment of ZIKV-infected cells reduced HMGB1 extracellular release in a dose-dependent manner and reduced > 90% virus titers. The antiviral effect, however, was not observed in resveratrol-treated HMGB1-knockdown cells, suggesting the importance of HMGB1 in the mechanism of resveratrol. Overall, our results suggested that the translocation of HMGB1 was increased during ZIKV infection and the inhibition of HMGB1 translocation by resveratrol reduced the ZIKV replication. The antiviral effects through the regulation of HMGB1 during ZIKV infection signify possible therapeutics treatments of the disease.

Biography:

Dr Chin Kim Ling graduated from Universiti Malaya in 2018 with a bachelor's degree in biomedical science (Hons). She then joined as a research assistant at the Department of Medical Microbiology, Universiti Malaya and Tropical Infectious Diseases Research & Education Centre, Universiti Malaya, where she had a great deal of control over the experimental design. She received her doctorate in 2022 at the same institution.

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