2nd Edition of World Congress on Infectious Diseases

June 17-18, 2022

June 17 -18, 2022 | Virtual Event
Infection 2022

Amit Kumar Kesharwani

Speaker at World Congress on Infectious Diseases 2022 - Amit Kumar Kesharwani
ICAR-Indian Agricultural Research Institute, India
Title : Whole-genome sequencing and comparative analysis of Indian race 4 of Xanthomonas campestris pv. campestris causing black rot disease in Brassica oleracea var. capitata

Abstract:

Xanthomonas campestris pv. campestris is a causative agent of black rot disease of cruciferous crops. A whole-genome sequence of any race of X. campestris pv. campestris has not been reported from India. The isolate Xcc-C7, race 4, of X. campestris pv. campestris was isolated from cabbage (Brassica oleracea var. capitata) from Bengaluru, in the southern parts of India. Whole-genome sequence data were generated by the next-generation sequencing based single-molecule real-time sequencing (SMRT) techniques. The complete genome assembly was composed of 5,121,051 bp and has a total GC content of 64.98%. Of 4,493 total genes, 4,347 CDS and 4,217 genes with assigned function were predicted in Xcc-C7 strain. The profiles of 146 RNA encoding genes were identified, including 6 ribosomal RNA-encoding genes, 54 transfer RNA encoding genes, and 86 noncoding RNAs. This whole-genome sequence data has been deposited in the NCBI GenBank database (CP077958). The comparative analysis of genomic features of Indian race 4 with genomes of four other strains (namely, X. campestris pv. campestris B100, 8004, CFBP5817, and ATCC33913) elucidates that the Indian race 4 is closely related to the Chilean strain CFBP5817. Furthermore, 19 genes of Xcc-C7 strain were identified as putative candidate genes that may play role in adaptation of cabbage as a host. found in common with genes of strain 8004, which were validated genes of pathogenicity. The phylogenetic tree of whole genome assemblies of the five strains showed that Xcc-C7 is closely related to CFBP5817, as compared with B100, ATCC33913, and 8004, suggesting that both strains CFBP5817 and Xcc-C7 belong to X. campestris pv. campestris race 4. This study will improve our knowledge of genomic diversity in X. campestris pv. campestris and pave the way for research on host–pathogen interactions (crucifer crops–X. campestris pv. campestris) to develop resistance in cultivated Brassicaceae crops.
What will audience learn from your presentation?
• The whole-genome sequence will be used to study the diversity, geographical changes and host specific development of pathogenicity genes in X. campestris pv. campestris.
• The outcomes of the study will be used to study the host-pathogen interaction to develop the resistance in Brassicaceae crops.
• The comparative analysis of genomic features of Indian race 4 of Xcc with other strains will also enhance the understanding of host specific development of disease in crucifer crops.
• Availability of genome resources of X. campestris pv. campestris from India.

Biography:

Mr. Amit Kumar Kesharwani Post-graduated in Microbiology from Gurukul Kangri Vishwavidyalaya, India in 2012. He has his expertise in microbiology, plant pathology and plant-microbe interaction. He then joined prestigious research laboratories in India to learn and gained experience in the field of Plant pathology and Biotechnology during 2012-2018. His research work enhances the understanding of bacterial pathogenesis in host. He has joined the research group of Dr. D. Singh at ICAR-Indian Agricultural Research Institute and Dr. A.S. Avasthi, Amity University, India to pursuing his Ph.D. degree in Biotechnology since 2018. He has published more than 6 research articles in SCI(E) journals & recipient of Junior and Young Scientist Awards.

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