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8th Edition of World Congress on Infectious Diseases

June 09-11, 2025 | Rome, Italy

June 09 -11, 2025 | Rome, Italy
Infection 2024

Carbapenemase-producing multidrug resistance of gram negative bacilli causing bacteremia at the general hospital of Mexico

Speaker at Infection Conferences - Silvia Giono Cerezo
Instituto Politecnico Nacional, Mexico
Title : Carbapenemase-producing multidrug resistance of gram negative bacilli causing bacteremia at the general hospital of Mexico

Abstract:

Bloodstream infections due to Gram-negative bacilli is a highly consequential nosocomial infection with serious consequences and even more so because the organisms are usually highly resistant to antimicrobials. The aims of this study were to describe incidence of bacteremia caused by Gram-negative ESKAPE bacteria during COVID-19 pandemic, the clinical and microbiological characteristics, and the Antimicrobial Resistance (AMR). A total of 115 Gram-negative ESKAPE isolates were collected from patients with nosocomial bacteremia in a tertiary care center in Mexico between January – December 2020. A. baumannii (34%) and K. pneumoniae (28%) were the most frequent followed by P. aeruginosa (23%) and Enterobacter spp (16%). A. baumannii showed the highest levels of AMR (100%), followed by K. pneumoniae (87%), Enterobacter spp (34%) and P. aeruginosa (20%). Furthermore, the 27 K. pneumoniae isolates resistant to beta-lactams carried the blaCTX-M-15 and blaTEM-1 genes, while 33/39 A. baumannii isolates were carriers of the blaTEM-1 gene. In isolates resistant to carbapenems, 29/39 A. baumannii were carriers of blaOXA-398 and 4 of blaOXA-24. In P. aeruginosa, only one isolate was a carrier of the blaVIM-2 gene, while the 2 K. pneumoniae and 1 Enterobacter spp carried the blaNDM gene. Among colistin-resistant isolates mcr-1 gene was not detected. The clonal relatedness assessed by Pulsed-Field Gel Electrophoresis (PFGE) and by Multi-Locus Sequence Typing (MLST) demonstrated two outbreaks caused by A. baumannii ST208 and ST369 and a genetic diversity for K. pneumoniae, P. aeruginosa and Enterobacter spp. Remarkably, the ST136 and ST208 belonged to the clonal complex CC92 and IC2. A. baumannii was associated with a high mortality rate of 72%, mainly in patients with COVID-19 75% cared for in the Respiratory Ward. There was no statistically significant association between MDR profile of Gram-negative bacteria and in COVID-19 and non-COVID-19 patients. In summary, the results points to the important role of Gram-negative MDR causing bacteremia in nosocomial settings before and during the COVID-19 epidemic. In addition, the COVID-19 pandemic had no local impact on antimicrobial resistance rates in the short term. Already publish Gram-negative ESKAPE bacteria bloodstream infections in patients during the COVID-19 pandemic.

Biography:

Dr Silvia Giono Cerezo PHD  National School of Biological Sciences  Instituto politécnico Nacional -IPN- Chemist Bacteriologist Parasitologist Doctor of Science Member of the National Academy of Medicine Member of the Mexican Association of Infectious Diseases Collegiate Member of the Postgraduate Studies Professor of Medical Bacteriology and Host-Parasite Relationship Theory and laboratory also Problems of Medical Bacteriology Tutor, Director Advisor of Bachelor's, Master'sand Doctorate students Lines of research: Diagnostic Medical Microbiology and Antimicrobial Resistance of the ESKAPE group Diagnosis and analysis of the genome of Helicobacter pylori MLST of Stenotrophomonas spp  several publications.

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