Title : RNA interference, its applications and benefits
Abstract:
The activation of double stranded interference RNA occurring in the host cell is a natural phenomenon to control the over transcriptional effects of genes that could suppress the translational patterns. This genetic ability is currently being exploited in making the medicines to suppress the lethal post- translational effects as an outcome of aberrant or disease related genes. This revelation has been made to treat those inherent diseases were rendered untreatable in the past. RNAi therapeutics silence the lethal/adverse effects of a gene function. The modulation of siRNA can be brought by changing some chemical patterns in RNA chains. The phosphorylation and off target effects are greatly reduced during phosphonate modifications, ribose sugar effects and base substitutions. Additionally, these modifications are likely to control the immune stimulation and hyper activation of the innate response. The universal pattern and advanced patterns have been used in various therapeutics. The heavy modifications in the anti-sense & sense strands are used using 2-OMe, L-DNA, UNA, N-acetyl glucosamine etc. Innate immune regulations can be established through TLR receptors’ signaling, but PKR and RIG are also capable to carry out the IFN induction in the absence of TLR sensors. This presentation will walk us through the immune modulation of siRNAs to reduce toxicity and regulate the innate and adaptive immune response to produce the favorable effects.
