Racha Boubekeur

Title : Systematic review of resistance to fluconazole for clinical isolates of the Candida albicans species diagnosed in the HCA parasitology mycology laboratory

Abstract:

The incidence of mycoses caused by Candida spp. has increased steadily over the past two decades, Candida albicans remains the most commonly isolated microscopic fungus. The objective of our study was to identify the profile of a few strains of Candida albicans isolated from different samples and to study resistance to Fluconazole over a period of 1 year from 2021 to 2022. The identification of 70 strains was done according to conventional methods, their sensitivity and resistance to antifungals was also evaluated by Vitek2 Semi-liquid Microdilution (AST-YS06 card) and FUNGITEST Bio Rad gallery. The antifungals tested were Amphotericin B, 5-fluorocytosine, azoles and echinochandins. The C. albicans strains had variable sensitivity: 100% to amphotericin B (MIC90 = 0.5 μg/mL); 99% to 5-fluorocytosine (MIC90 = 4 μg/mL); 86.7% to voriconazole (MIC50 = 0.06 μg/mL) and 80% to fluconazole (MIC50 = 2 μg/mL and MIC90 = 32 μg/m). In addition to the intrinsic resistance of the species studied, we noted the emergence of acquired resistance including resistance to some azoles Fluconazole (n=5), Itraconazol (n=4), Miconazole (n=5), Ketoconazole (n=2), and Voriconazole (n=1). Our study showed that C. albicans was the most incriminated strain. However, an emergence of acquired resistance is starting, hence the importance of carrying out an antifungigram in order to monitor these resistance profiles.

Biography:

Doctor BOUBEKEUR Racha obtained her scientific baccalaureate in 2006, and joined the Houari Boumédienne University of Science and Technology in Algeria, where she obtained her bachelor's degree in Parasitology, Myclogy and Health, followed in 2011 by a Master's degree in Parasitology, Biology, Ecology and Environment, having studied food-borne parasitosis. In 2012, she joined the Medical Parasitology and Mycology Laboratory at the Army Central Hospital, where she worked in the Mycology Unit and the Molecular Biology Unit, participating in the diagnosis and development of new techniques using molecular biology tools, and taking part in a number of scientific projects. In 2016, she published the first molecular characterization of Candida africana in Algeria, followed by several publications in the field of Parasitology and Mycology. In 2017, she joined the University of Bejaia Abderahmane Mira in Algeria, where she worked in the Applied Zoology Laboratory on her doctoral thesis, entitled biological and parasitological study of Mugilidae from the Gulf of Bejaia. After a number of publications, she obtained her doctorate in 2022, and joined the Parsitology-Mycology Laboratory at the Army Central Hospital.