The problems of infections transmitted by sucking a tick are due to the expansion of the distribution area of ticks and an increase in the incidence of tick-borne infections. Farm animals that make up the agrobiodiversity of Uzbekistan are at risk of disease with numerous pathogens - bacteria and viruses, the carriers of which are blood-sucking mites of the Ixodidae family. They are an extensive group of ectoparasites, quite widespread in terrestrial cenoses, which cause significant damage to the livestock and poultry sector - on the one hand, and being carriers of human pathogens (anthropozoonoses) adversely affect human health - on the other hand. In general, these problems are among the serious issues of socio-economic and environmental significance. People seeking medical help after a tick bite is increasing every year. At the same time, diagnosing tick-borne infections causes difficulties for practitioners.
One thousand five hundred seventeen tick specimens were collected and studied in the Samarkand region. The field material was delivered to the Laboratory of Natural Focal Particularly Dangerous Virus Infections (POOOVI) of the Research Institute of Virology of Uzbekistan. The material was delivered to the laboratory within five days after collection in compliance with the rules of biological safety. These samples were registered, systematized, and sorted by species. The resulting suspension of ticks was stored until the start of the study at T - 200C.
Tick suspension samples were tested for rickettsia using test systems for PCR AmpliSense Coxiella burnetii – FL, AmpliSense TBEV, B.burgdorferi sl, A.phagocytophilum, E.chaffensis/E.muris - FL, Vector Best RealBest Extraction 100, Vector Best RealBest Rickettsia species, Vector Best RealBest Rickettsia sibirica/R.heilongjiangensis.
As a result of the research, genetic material was found in ticks to the following infections: Rickettsia species - Rhipicephalus turanicus, Boophilus calcaratus, Hyalomma detritum, H anatolicum - Coxiella burnetii, collected in the Samarkand region - H.detritum. Detection and identification of pathogens of various infections are essential to prevent outbreaks and the emergence of new foci of tick-borne diseases in Uzbekistan.