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4th Edition of World Congress on Infectious Diseases

June 21-22, 2023 | Rome, Italy

June 21 -22, 2023 | Rome, Italy
Infection 2023

Ali Mirzapour

Speaker at World Congress on Infectious Diseases 2023 - Ali Mirzapour
Islamic Azad University, Iran (Islamic Republic of)
Title : Genetic characterization and frequency of T. gondii infection in stray cats in lorestan province, West of Iran


Background: Cats as the definitive hosts of Toxoplasma gondii play an important role in parasite dispersion and outbreaks. To understand the transmission pattern of this parasite, the distribution of genetic diversity of T. gondii in cats is important. The current study aimed to determine the prevalence and genetic diversity of T. gondii isolates from stray cats in Lorestan Province, western Iran.

Materials and Methods: Fresh stool specimens were collected from 200 cats in Lorestan province, West of Iran during 2021-2022. Formalin-ether concentration technique and the modified acid-fast staining method were used for the recognition of oocysts. The DNA was extracted from the stool by a commercial Genomic Mini Kit (Bioneer, South Korea). PCR-RFLP examination, sequencing, and phylogenetic analysis of the GRA6 target gene were used to determine the genetic characterization of T. gondii.

Results:  All samples were negative by parasitology techniques. Thirteen (6.5%) samples were positive by using the GRA6-PCR assay. The results of PCR-RFLP indicated that all samples were belonging to the type III of T. gondii genotype. The phylogenic tree for GRA6 nucleotide sequences of 2 isolates from current work, with 12 reference strains of T. gondii and one strain of a Hammondia hamondi as external control indicated a 5% nucleotide difference.

Conclusions: According to the results, high sensitivity and specificity, molecular tests are suitable for genotyping and the results of the RFLP technique indicated that type III of T. gondii is the most important and predominant genotype in Lorestan Province, western Iran.

Keywords: Genetic characterization, T. gondii, Stray cats, Lorestan province, PCR-RFLP

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I'm Dr. Ali Mirzapour studied Microbiology at the Tehran Center of Science and Research University University, Iran and graduated as MS in 2012. received her PhD degree in 2017 at the Medical parasitology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

I have published more than 16 research articles in ISI journals.

I'm married, I have two children.